Q4. The next step is to measure multiple properties of individual particles

Q4. What would we like to know? What are the most promising techniques for doing so? What will we do with the data?

George says (http://wp.me/p7ffKH-B): Way back when, Vern Asper measured both particle size and settling velocity on the same particles. More recently, Andrew McD has been measuring particle concentrations and fluxes on two different sets of particles and using the results to infer velocity as a function of size. Is there a way to measure both properties on the same particles so that we could perform more sophisticated analyses?

Q3. How do we calibrate different instruments?

My idea: ideally in two steps.

  1. On shore, using a tank filled with balls of a known size (or size range/consecutively different sizes)
  2. At sea, mounted on the same frame

Can we come up with calibration protocols?

#calibration

Q2. How do we convert between measurements made by different instruments?

Further to George’s comment (http://wp.me/p7ffKH-B). This is one of the central questions I would like this working group to resolve. It starts with the simple question: What is #esd? Different people have different ideas about how to extrapolate their data into a 3D object. For example, I calculated ESD for each particle assuming faecal pellets are cylindrical and all other particles are prolate ellipsoids. But is that what everyone does? And how do you cope with aggregates that are made of different things stuck together? For example:

mixed aggregate

Further to that, how do you determine where a particle ends? I suspect most of us use automatic algorithms, but again, it would be important to make sure that we all use the same threshold. When dealing with 3D objects, even a small error in estimated ESD will have a large effect on final volume (e.g. POC content) calculations!

Maybe we can come up with definitions for each device, all of which are internally consistent?

#1xbet, #2009-062, #432, #%d0%b0%d0%bd%d0%b0%d0%bb%d0%b8%d1%82%d0%b8%d0%ba%d0%b0, #%d0%bb%d0%b8%d0%b3%d0%b0%d1%87%d0%b5%d0%bc%d0%bf%d0%b8%d0%be%d0%bd%d0%be%d0%b2, #%d0%bf%d1%80%d0%be%d0%b3%d0%bd%d0%be%d0%b7%d1%8b, #mutations, #patients

Q1. How do we determine/distinguish the distributions of different objects from the different instruments?

And how does the certainty vary with object size?

Further to George’s comment (http://wp.me/p7ffKH-B). I think Sünnje and Klas have compared the LOPC and VPR data to distinguish marine snow and zooplankton, and it was pretty problematic. I think George’s idea to analyze and interpret the same data set using different view points is great: 1. ‘particle objects’ are zooplankton, 2. ‘particle objects’ are marine snow.

Just for fun, I’ve attached a couple of photos taken with a camera (particles from the MSC) and the VPR (courtesy of Klas!) . These highlight nicely the diversity we should expect.

Marine snow_small copy

vpr3

Share your thoughts

Hi all,

I thought it would be good to have a platform, where we can write down ideas and comment on these. For example future directions, updates, definitions, problems, etc.

You can tag your post to help structuring the discussion. For example, use #esd if you want to discuss how to define particle size.

You can also address a particular member using the ‘at’ symbol. For example: ‘@saristar, this is awesome!’

Obviously, this website is not finished yet and will probably only serve as a mockup until we have the final website hosted on the noc server. This will then also include the database search and submission functions.

Until then, I will make anyone interested an admin, so that you can join the discussion, and add and alter the website. We need details for all devices and definitions/recommendations!

Let me know what you think!
Sari